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review: expert reviews, ratings, and advice find the perfect mac.Episode notes Simon Helberg joins Max and Jessica to talk about his new show Man Seeking Woman, why he got into comedy in the first place, and the time he played Dr. Strange. This episode is sponsored by Fuzzy Door. Click here to learn more and save $50 on your next hotel. Website: FuzzyDoor.com Get Tickets: fuzzydoor.com/podcast You can follow Max on Twitter, and he’ll be live Tweeting the show starting at 11:30 a.m. Eastern.Effects of elastase-induced emphysema on the synthesis and surface expression of the interleukin-4 receptor in lung macrophages. Exposure to the protease elastase can induce emphysema in rats and is characterized by macrophage activation. We have investigated the effects of elastase-induced emphysema on the synthesis and surface expression of the interleukin-4 receptor (IL-4R) in lung macrophages. Rats were exposed to 1 U of elastase for 12 weeks and control animals received PBS. Lung macrophages were purified and maintained in culture for 24 h. Cell activation was induced with lipopolysaccharide (LPS) or interferon-gamma (IFN-gamma). IL-4R mRNA was quantified by a competitive polymerase chain reaction assay. Surface expression of IL-4R was determined by FACS analysis. Elastase exposure increased the amount of IL-4R mRNA in cells stimulated with LPS or IFN-gamma. In contrast, total cellular IL-4R expression was not affected. This increase in IL-4R mRNA after elastase exposure was specific to the macrophages because IL-4R levels in bronchial epithelial cells were not affected. Increased IL-4R mRNA in lung macrophages could be related to the increased number of activated alveolar macrophages in emphysematous rats and/or increased production of IL-4 by macrophages in response to elastase exposure. In addition, this increase may be important for the regulation of inflammatory responses, such as the up-regulation of the IL-4R, in macrophages in emphysema.Q: Why can't I be reached by domain
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